| 1 | Fri | 2025-08-01 | 20:00 | Luton Town | 1–0 | AFC Wimbledon | 11,735 | Kenilworth Road Stadium | Thomas Parsons | Match Report | |
| 1 | Sat | 2025-08-02 | 12:30 | Cardiff City | 2–1 | Peterborough United | 19,615 | Cardiff City Stadium | Ben Speedie | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Bradford City | 2–1 | Wycombe Wanderers | 19,604 | University of Bradford Stadium | Seb Stockbridge | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Plymouth Argyle | 1–3 | Barnsley | 16,483 | Home Park | Declan Bourne | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Huddersfield Town | 3–0 | Leyton Orient | 17,062 | Accu Stadium | Simon Mather | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Blackpool | 2–3 | Stevenage | 10,090 | Bloomfield Road Stadium | Matthew Corlett | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Burton Albion | 2–1 | Mansfield Town | 5,170 | Pirelli Stadium | Edward Duckworth | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Lincoln City | 2–0 | Reading | 9,054 | LNER Stadium | Martin Woods | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Rotherham United | 2–1 | Port Vale | 9,883 | AESSEAL New York Stadium | Jacob Miles | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Doncaster Rovers | 1–0 | Exeter City | 8,688 | Eco-Power Stadium | Ross Joyce | Match Report | |
| 1 | Sat | 2025-08-02 | 15:00 | Wigan Athletic | 3–1 | Northampton Town | 9,471 | The Brick Community Stadium | Alex Chilowicz | Match Report | |
| 1 | Sun | 2025-08-03 | 12:00 | Stockport | 2–0 | Bolton Wanderers | 10,172 | Edgeley Park | Elliot Bell | Match Report | |
| | | | | | | | | | | |
| 2 | Thu | 2025-08-07 | 20:00 | Port Vale | 0–0 | Cardiff City | 9,791 | Vale Park | Tom Reeves | Match Report | |
| 2 | Sat | 2025-08-09 | 12:30 | Reading | 0–2 | Huddersfield Town | 12,538 | Select Car Leasing Stadium | Matthew Corlett | Match Report | |
| 2 | Sat | 2025-08-09 | 12:30 | Peterborough United | 0–2 | Luton Town | 10,827 | Weston Homes Stadium | Scott Oldham | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Stevenage | 1–0 | Rotherham United | 3,470 | The Lamex Stadium | James Durkin | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Barnsley | 3–2 | Burton Albion | 12,456 | Oakwell Stadium | David Rock | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | AFC Wimbledon | 2–0 | Lincoln City | 8,067 | The Cherry Red Records Stadium | Matthew Russell | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Wycombe Wanderers | 1–2 | Stockport | 5,590 | Adams Park | Oliver Yates | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Bolton Wanderers | 2–0 | Plymouth Argyle | 21,570 | Toughsheet Community Stadium | Ross Joyce | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Exeter City | 4–1 | Blackpool | 6,852 | St James Park | Carl Brook | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Leyton Orient | 2–0 | Wigan Athletic | 8,111 | The BetWright Stadium | Neil Hair | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Northampton Town | 0–0 | Bradford City | 7,295 | Sixfields Stadium | Simon Mather | Match Report | |
| 2 | Sat | 2025-08-09 | 15:00 | Mansfield Town | 1–2 | Doncaster Rovers | 8,798 | One Call Stadium | Andrew Humphries | Match Report | |
| | | | | | | | | | | |
| 3 | Sat | 2025-08-16 | 12:30 | Burton Albion | 0–0 | Port Vale | 4,404 | Pirelli Stadium | Scott Oldham | Match Report | |
| 3 | Sat | 2025-08-16 | 12:30 | Exeter City | 1–2 | Mansfield Town | 6,166 | St James Park | Oliver Yates | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Bradford City | 2–1 | Luton Town | 20,233 | University of Bradford Stadium | Edward Duckworth | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Doncaster Rovers | 1–1 | Wycombe Wanderers | 8,084 | Eco-Power Stadium | Martin Coy | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Barnsley | 1–1 | Bolton Wanderers | 13,167 | Oakwell Stadium | Leigh Doughty | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Reading | 1–2 | AFC Wimbledon | 13,757 | Select Car Leasing Stadium | Jamie O'Connor | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Stevenage | 2–0 | Northampton Town | 3,350 | The Lamex Stadium | Tom Reeves | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Blackpool | 3–2 | Huddersfield Town | 10,922 | Bloomfield Road Stadium | Elliot Bell | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Leyton Orient | 2–2 | Stockport | 7,651 | The BetWright Stadium | Ruebyn Ricardo | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Wigan Athletic | 2–0 | Peterborough United | 8,748 | The Brick Community Stadium | Stephen Parkinson | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Cardiff City | 3–0 | Rotherham United | 17,555 | Cardiff City Stadium | Thomas Parsons | Match Report | |
| 3 | Sat | 2025-08-16 | 15:00 | Lincoln City | 3–2 | Plymouth Argyle | 8,503 | LNER Stadium | Matthew Russell | Match Report | |
| | | | | | | | | | | |
| 4 | Tue | 2025-08-19 | 19:45 | Stockport | 1–2 | Bradford City | 9,641 | Edgeley Park | Thomas Parsons | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Wycombe Wanderers | 0–1 | Exeter City | 4,661 | Adams Park | Ben Atkinson | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | AFC Wimbledon | 0–1 | Cardiff City | 8,452 | The Cherry Red Records Stadium | David Rock | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Northampton Town | 0–1 | Lincoln City | 5,729 | Sixfields Stadium | Oliver Yates | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Peterborough United | 0–1 | Barnsley | 7,148 | Weston Homes Stadium | Scott Jackson | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Luton Town | 1–0 | Wigan Athletic | 10,751 | Kenilworth Road Stadium | Carl Brook | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Huddersfield Town | 2–0 | Doncaster Rovers | 18,529 | Accu Stadium | Leigh Doughty | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Port Vale | 1–2 | Stevenage | 6,653 | Vale Park | Seb Stockbridge | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Mansfield Town | 2–0 | Blackpool | 7,574 | One Call Stadium | Ben Toner | Match Report | |
| 4 | Tue | 2025-08-19 | 19:45 | Plymouth Argyle | 0–1 | Leyton Orient | 15,422 | Home Park | Ben Speedie | Match Report | |
| 4 | Wed | 2025-08-20 | 20:00 | Bolton Wanderers | 1–1 | Reading | 19,625 | Toughsheet Community Stadium | Tom Reeves | Match Report | |
| | | | | | | | | | | |
| 5 | Sat | 2025-08-23 | 12:30 | Rotherham United | 2–2 | Wigan Athletic | 8,200 | AESSEAL New York Stadium | Oliver Langford | Match Report | |
| 5 | Sat | 2025-08-23 | 12:30 | Luton Town | 0–1 | Cardiff City | 11,456 | Kenilworth Road Stadium | Martin Coy | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Wycombe Wanderers | 2–2 | Reading | 6,639 | Adams Park | Alex Chilowicz | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Huddersfield Town | 1–0 | Stevenage | 16,489 | Accu Stadium | Darren Drysdale | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Port Vale | 0–1 | Doncaster Rovers | 7,728 | Vale Park | Ruebyn Ricardo | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Peterborough United | 1–1 | Bradford City | 8,874 | Weston Homes Stadium | James Durkin | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Bolton Wanderers | 1–1 | Lincoln City | 19,938 | Toughsheet Community Stadium | Jacob Miles | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Plymouth Argyle | 1–0 | Blackpool | 15,322 | Home Park | David Rock | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Stockport | 2–1 | Burton Albion | 8,649 | Edgeley Park | Seb Stockbridge | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Northampton Town | 2–0 | Exeter City | 5,739 | Sixfields Stadium | Edward Duckworth | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | Mansfield Town | 4–1 | Leyton Orient | 7,537 | One Call Stadium | Carl Brook | Match Report | |
| 5 | Sat | 2025-08-23 | 15:00 | AFC Wimbledon | 2–0 | Barnsley | 8,361 | The Cherry Red Records Stadium | Oliver Yates | Match Report | |
| | | | | | | | | | | |
| 6 | Sat | 2025-08-30 | 12:30 | Cardiff City | 4–0 | Plymouth Argyle | 21,813 | Cardiff City Stadium | Scott Oldham | Match Report | |
| 6 | Sat | 2025-08-30 | 12:30 | Doncaster Rovers | 1–0 | Rotherham United | 1,957 | Eco-Power Stadium | Elliot Bell | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Blackpool | 1–1 | Bolton Wanderers | 11,579 | Bloomfield Road Stadium | Tom Nield | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Leyton Orient | 0–1 | Northampton Town | 8,009 | The BetWright Stadium | Jacob Miles | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Wigan Athletic | 1–1 | Stockport | 11,078 | The Brick Community Stadium | Ross Joyce | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Stevenage | 1–0 | Wycombe Wanderers | 3,779 | The Lamex Stadium | Simon Mather | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Barnsley | 3–1 | Huddersfield Town | 15,783 | Oakwell Stadium | Edward Duckworth | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Exeter City | 3–0 | Peterborough United | 6,239 | St James Park | Matthew Corlett | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Reading | 1–0 | Port Vale | 12,624 | Select Car Leasing Stadium | Thomas Parsons | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Burton Albion | 0–3 | Luton Town | 4,487 | Pirelli Stadium | Peter Wright | Match Report | |
| 6 | Sat | 2025-08-30 | 15:00 | Bradford City | 3–2 | AFC Wimbledon | 19,076 | University of Bradford Stadium | Martin Coy | Match Report | |
| 6 | Sat | 2025-08-30 | 15:15 | Lincoln City | 1–1 | Mansfield Town | 9,626 | LNER Stadium | Aaron Bannister | Match Report | |
| | | | | | | | | | | |
| 7 | Sat | 2025-09-06 | 15:00 | Doncaster Rovers | 3–1 | Bradford City | 11,191 | Eco-Power Stadium | Alex Chilowicz | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Wycombe Wanderers | 2–0 | Mansfield Town | 5,080 | Adams Park | Scott Oldham | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Rotherham United | 1–0 | Exeter City | 7,699 | AESSEAL New York Stadium | Martin Woods | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Port Vale | 2–3 | Leyton Orient | 7,721 | Vale Park | Ross Joyce | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Lincoln City | 2–2 | Wigan Athletic | 8,208 | LNER Stadium | Alan Young | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Plymouth Argyle | 4–2 | Stockport | 15,719 | Home Park | Neil Hair | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Bolton Wanderers | 3–0 | AFC Wimbledon | 20,157 | Toughsheet Community Stadium | Darren Drysdale | Match Report | |
| 7 | Sat | 2025-09-06 | 15:00 | Huddersfield Town | 3–2 | Peterborough United | 16,383 | Accu Stadium | Martin Coy | Match Report | |
| | | | | | | | | | | |
| 8 | Sat | 2025-09-13 | 12:30 | Bradford City | 3–1 | Huddersfield Town | 24,075 | University of Bradford Stadium | Scott Oldham | Match Report | |
| 8 | Sat | 2025-09-13 | 12:30 | Northampton Town | 1–0 | Blackpool | 6,058 | Sixfields Stadium | Seb Stockbridge | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Leyton Orient | 1–1 | Bolton Wanderers | 8,731 | The BetWright Stadium | David Rock | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Wigan Athletic | 3–0 | Doncaster Rovers | 9,288 | The Brick Community Stadium | Craig Hicks | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Peterborough United | 2–1 | Wycombe Wanderers | 8,595 | Weston Homes Stadium | Jacob Miles | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Barnsley | 3–2 | Reading | 10,803 | Oakwell Stadium | Elliot Bell | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Luton Town | 2–3 | Plymouth Argyle | 11,801 | Kenilworth Road Stadium | Oliver Yates | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | AFC Wimbledon | 2–1 | Rotherham United | 8,058 | The Cherry Red Records Stadium | Lee Swabey | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Stockport | 1–1 | Cardiff City | 9,956 | Edgeley Park | Simon Mather | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Exeter City | 0–2 | Port Vale | 6,221 | St James Park | Matthew Russell | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Mansfield Town | 1–1 | Stevenage | 7,301 | One Call Stadium | Martin Woods | Match Report | |
| 8 | Sat | 2025-09-13 | 15:00 | Burton Albion | 0–1 | Lincoln City | 3,392 | Pirelli Stadium | Matthew Corlett | Match Report | |
| | | | | | | | | | | |
| 9 | Sat | 2025-09-20 | 12:30 | Blackpool | 1–0 | Barnsley | 10,529 | Bloomfield Road Stadium | Martin Coy | Match Report | |
| 9 | Sat | 2025-09-20 | 12:30 | Bolton Wanderers | 4–1 | Wigan Athletic | 25,565 | Toughsheet Community Stadium | Ben Speedie | Match Report | |
| 9 | Sat | 2025-09-20 | 12:30 | Lincoln City | 3–1 | Luton Town | 9,255 | LNER Stadium | Seb Stockbridge | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Port Vale | 2–1 | Mansfield Town | 9,013 | Vale Park | Alex Chilowicz | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Wycombe Wanderers | 2–0 | Northampton Town | 5,197 | Adams Park | Andy Madley | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Rotherham United | 0–1 | Stockport | 8,784 | AESSEAL New York Stadium | David Rock | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Stevenage | 2–1 | Exeter City | 4,129 | The Lamex Stadium | Craig Hicks | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Reading | 2–1 | Leyton Orient | 12,553 | Select Car Leasing Stadium | Oliver Yates | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Doncaster Rovers | 1–2 | AFC Wimbledon | 7,889 | Eco-Power Stadium | Peter Wright | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Huddersfield Town | 0–0 | Burton Albion | 15,654 | Accu Stadium | Andrew Humphries | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Plymouth Argyle | 0–1 | Peterborough United | 15,994 | Home Park | James Linington | Match Report | |
| 9 | Sat | 2025-09-20 | 15:00 | Cardiff City | 1–3 | Bradford City | 22,132 | Cardiff City Stadium | Martin Woods | Match Report | |
| | | | | | | | | | | |
| 10 | Sat | 2025-09-27 | 12:30 | AFC Wimbledon | 2–1 | Wycombe Wanderers | 8,477 | The Cherry Red Records Stadium | Scott Jackson | Match Report | |
| 10 | Sat | 2025-09-27 | 12:30 | Leyton Orient | 2–3 | Stevenage | 7,898 | The BetWright Stadium | Andrew Humphries | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Wigan Athletic | 0–2 | Cardiff City | 10,609 | The Brick Community Stadium | Darren Drysdale | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Burton Albion | 0–4 | Plymouth Argyle | 4,271 | Pirelli Stadium | Tom Reeves | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Bradford City | 1–0 | Blackpool | 21,531 | University of Bradford Stadium | Ben Atkinson | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Mansfield Town | 2–1 | Rotherham United | 8,201 | One Call Stadium | Simon Mather | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Luton Town | 1–0 | Doncaster Rovers | 10,957 | Kenilworth Road Stadium | Tim Robinson | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Northampton Town | 2–0 | Bolton Wanderers | 7,366 | Sixfields Stadium | Scott Oldham | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Barnsley | 0–2 | Port Vale | 10,688 | Oakwell Stadium | James Linington | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Peterborough United | 0–3 | Lincoln City | 9,943 | Weston Homes Stadium | Thomas Parsons | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Exeter City | 0–1 | Huddersfield Town | 6,477 | St James Park | David Rock | Match Report | |
| 10 | Sat | 2025-09-27 | 15:00 | Stockport | 1–1 | Reading | 9,249 | Edgeley Park | Oliver Mackey | Match Report | |
| | | | | | | | | | | |
| 7 | Tue | 2025-09-30 | 19:45 | Blackpool | 2–2 | Luton Town | 8,513 | Bloomfield Road Stadium | Martin Woods | Match Report | |
| 7 | Tue | 2025-09-30 | 19:45 | Cardiff City | 0–1 | Burton Albion | 15,814 | Cardiff City Stadium | Jacob Miles | Match Report | |
| | | | | | | | | | | |
| 11 | Thu | 2025-10-02 | 20:00 | Rotherham United | 2–2 | Bradford City | 9,546 | AESSEAL New York Stadium | Oliver Yates | Match Report | |
| 11 | Sat | 2025-10-04 | 12:30 | Wycombe Wanderers | 2–2 | Barnsley | 4,932 | Adams Park | Ross Joyce | Match Report | |
| 11 | Sat | 2025-10-04 | 12:30 | Doncaster Rovers | 1–1 | Burton Albion | 7,435 | Eco-Power Stadium | Thomas Parsons | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Port Vale | 0–0 | Northampton Town | 7,657 | Vale Park | Martin Woods | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Lincoln City | 0–1 | Exeter City | 9,053 | LNER Stadium | Andrew Humphries | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Blackpool | 0–2 | AFC Wimbledon | 8,906 | Bloomfield Road Stadium | Ben Speedie | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Bolton Wanderers | 2–1 | Peterborough United | 20,336 | Toughsheet Community Stadium | Seb Stockbridge | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Huddersfield Town | 1–2 | Stockport | 18,707 | Accu Stadium | Ben Toner | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Reading | 1–1 | Mansfield Town | 12,328 | Select Car Leasing Stadium | Paul Tierney | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Cardiff City | 4–3 | Leyton Orient | 18,528 | Cardiff City Stadium | Matthew Corlett | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Plymouth Argyle | 1–1 | Wigan Athletic | 16,062 | Home Park | Ben Atkinson | Match Report | |
| 11 | Sat | 2025-10-04 | 15:00 | Stevenage | 2–0 | Luton Town | 7,228 | The Lamex Stadium | James Bell | Match Report | |
| | | | | | | | | | | |
| 12 | Sat | 2025-10-11 | 14:00 | Northampton Town | 1–2 | Rotherham United | 6,548 | Sixfields Stadium | Paul Tierney | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | AFC Wimbledon | 1–1 | Port Vale | 8,686 | The Cherry Red Records Stadium | Carl Brook | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | Stockport | 1–0 | Blackpool | 10,091 | Edgeley Park | Jacob Miles | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | Leyton Orient | 4–0 | Doncaster Rovers | 8,128 | The BetWright Stadium | Edward Duckworth | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | Wigan Athletic | 0–1 | Wycombe Wanderers | 8,546 | The Brick Community Stadium | Andrew Humphries | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | Burton Albion | 3–0 | Bolton Wanderers | 4,725 | Pirelli Stadium | Neil Hair | Match Report | |
| 12 | Sat | 2025-10-11 | 15:00 | Exeter City | 1–1 | Reading | 7,361 | St James Park | James Durkin | Match Report | |
| | | | | | | | | | | |
| 13 | Thu | 2025-10-16 | 20:00 | Huddersfield Town | 1–2 | Bolton Wanderers | 17,201 | Accu Stadium | Oliver Yates | Match Report | |
| 13 | Sat | 2025-10-18 | 12:30 | Lincoln City | 1–0 | Stevenage | 8,451 | LNER Stadium | Martin Coy | Match Report | |
| 13 | Sat | 2025-10-18 | 12:30 | Burton Albion | 0–1 | Peterborough United | 3,786 | Pirelli Stadium | Elliot Bell | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Cardiff City | 2–1 | Reading | 20,363 | Cardiff City Stadium | Lee Swabey | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Doncaster Rovers | 1–2 | Northampton Town | 8,113 | Eco-Power Stadium | John Busby | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Plymouth Argyle | 1–2 | AFC Wimbledon | 16,215 | Home Park | Matthew Corlett | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Wigan Athletic | 1–0 | Port Vale | 10,932 | The Brick Community Stadium | Ross Martin | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Luton Town | 0–2 | Mansfield Town | 11,784 | Kenilworth Road Stadium | Ross Joyce | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Bradford City | 2–2 | Barnsley | 22,228 | University of Bradford Stadium | Andrew Humphries | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Stockport | 1–0 | Exeter City | 9,190 | Edgeley Park | Aaron Bannister | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Blackpool | 1–1 | Wycombe Wanderers | 8,876 | Bloomfield Road Stadium | Neil Hair | Match Report | |
| 13 | Sat | 2025-10-18 | 15:00 | Rotherham United | 1–0 | Leyton Orient | 8,057 | AESSEAL New York Stadium | Scott Oldham | Match Report | |
| | | | | | | | | | | |
| 7 | Tue | 2025-10-21 | 20:00 | Reading | 1–0 | Northampton Town | 9,228 | Select Car Leasing Stadium | Abigail Byrne | Match Report | |
| | | | | | | | | | | |
| 14 | Thu | 2025-10-23 | 20:00 | Exeter City | 2–0 | Plymouth Argyle | 8,262 | St James Park | Alex Chilowicz | Match Report | |
| 14 | Sat | 2025-10-25 | 12:30 | Bolton Wanderers | 1–0 | Cardiff City | 22,587 | Toughsheet Community Stadium | Ben Toner | Match Report | |
| 14 | Sat | 2025-10-25 | 12:30 | Mansfield Town | 1–1 | Wigan Athletic | 7,598 | One Call Stadium | Seb Stockbridge | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Reading | 1–1 | Doncaster Rovers | 12,003 | Select Car Leasing Stadium | Matthew Russell | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Stevenage | 1–1 | Bradford City | 5,279 | The Lamex Stadium | Samuel Allison | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Peterborough United | 1–2 | Blackpool | 8,315 | Weston Homes Stadium | Sam Mulhall | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Wycombe Wanderers | 3–0 | Huddersfield Town | 5,738 | Adams Park | Lee Swabey | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Leyton Orient | 1–0 | Lincoln City | 7,814 | The BetWright Stadium | Stephen Parkinson | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Barnsley | 0–1 | Rotherham United | 12,468 | Oakwell Stadium | Ben Speedie | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | AFC Wimbledon | 0–1 | Burton Albion | 8,361 | The Cherry Red Records Stadium | Ben Atkinson | Match Report | |
| 14 | Sat | 2025-10-25 | 15:00 | Northampton Town | 0–1 | Luton Town | 8,029 | Sixfields Stadium | Matthew Corlett | Match Report | |
| 14 | Mon | 2025-10-27 | 20:00 | Port Vale | 0–3 | Stockport | 8,452 | Vale Park | Edward Duckworth | Match Report | |
| | | | | | | | | | | |
| 12 | Tue | 2025-10-28 | 19:45 | Bradford City | 0–0 | Lincoln City | 19,787 | University of Bradford Stadium | Elliot Bell | Match Report | |
| 12 | Tue | 2025-10-28 | 19:45 | Mansfield Town | 2–0 | Plymouth Argyle | 7,705 | One Call Stadium | Matthew Russell | Match Report | |
| | | | | | | | | | | |
| 4 | Tue | 2025-11-04 | 19:45 | Rotherham United | 2–2 | Burton Albion | 7,228 | AESSEAL New York Stadium | Ross Joyce | Match Report | |
| | | | | | | | | | | |
| 15 | Thu | 2025-11-06 | 20:00 | Reading | 1–0 | Stevenage | 9,233 | Select Car Leasing Stadium | Ben Speedie | Match Report | |
| 15 | Sat | 2025-11-08 | 12:30 | Wycombe Wanderers | 4–1 | Leyton Orient | 4,898 | Adams Park | Martin Woods | Match Report | |
| 15 | Sat | 2025-11-08 | 12:30 | Northampton Town | 2–1 | Mansfield Town | 6,849 | Sixfields Stadium | Elliot Bell | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Rotherham United | 3–0 | Lincoln City | 8,912 | AESSEAL New York Stadium | Alex Chilowicz | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Blackpool | 3–1 | Cardiff City | 10,792 | Bloomfield Road Stadium | Robert Madley | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Peterborough United | 5–0 | AFC Wimbledon | 9,287 | Weston Homes Stadium | Simon Mather | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Bolton Wanderers | 4–0 | Port Vale | 21,658 | Toughsheet Community Stadium | Martin Coy | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Bradford City | 1–2 | Burton Albion | 20,508 | University of Bradford Stadium | Craig Hicks | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Exeter City | 1–1 | Wigan Athletic | 6,585 | St James Park | Stephen Parkinson | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Stockport | 0–3 | Luton Town | 10,090 | Edgeley Park | Joshua Smith | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Huddersfield Town | 3–1 | Plymouth Argyle | 16,235 | Accu Stadium | Seb Stockbridge | Match Report | |
| 15 | Sat | 2025-11-08 | 15:00 | Doncaster Rovers | 1–2 | Barnsley | 11,415 | Eco-Power Stadium | Darren Drysdale | Match Report | |
| | | | | | | | | | | |
| 16 | Sat | 2025-11-15 | 15:00 | Leyton Orient | 2–1 | Exeter City | 8,519 | The BetWright Stadium | Ruebyn Ricardo | Match Report | |
| 16 | Sat | 2025-11-15 | 15:00 | Lincoln City | 2–1 | Doncaster Rovers | 9,812 | LNER Stadium | David Rock | Match Report | |
| 16 | Sat | 2025-11-15 | 15:00 | Burton Albion | 1–0 | Blackpool | 4,054 | Pirelli Stadium | Lee Swabey | Match Report | |
| 16 | Sat | 2025-11-15 | 15:00 | Luton Town | 0–0 | Rotherham United | 11,091 | Kenilworth Road Stadium | Craig Hicks | Match Report | |
| 16 | Sat | 2025-11-15 | 15:00 | Port Vale | 0–0 | Wycombe Wanderers | 6,783 | Vale Park | Jacob Miles | Match Report | |
| | | | | | | | | | | |
| 17 | Thu | 2025-11-20 | 20:00 | Peterborough United | 3–0 | Stockport | 6,681 | Weston Homes Stadium | Matthew Russell | Match Report | |
| 17 | Sat | 2025-11-22 | 12:30 | Port Vale | 0–1 | Plymouth Argyle | 7,358 | Vale Park | Abigail Byrne | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Northampton Town | 1–3 | Cardiff City | 7,151 | Sixfields Stadium | Neil Hair | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Reading | 1–1 | Rotherham United | 15,279 | Select Car Leasing Stadium | Alan Young | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Barnsley | 5–0 | Luton Town | 10,882 | Oakwell Stadium | Thomas Parsons | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Mansfield Town | 1–3 | Huddersfield Town | 8,590 | One Call Stadium | Craig Hicks | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | AFC Wimbledon | 1–2 | Wigan Athletic | 8,370 | The Cherry Red Records Stadium | Richard Eley | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Wycombe Wanderers | 3–2 | Lincoln City | 4,872 | Adams Park | Oliver Langford | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Leyton Orient | 1–1 | Blackpool | 8,181 | The BetWright Stadium | Samuel Allison | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Bolton Wanderers | 0–0 | Bradford City | 26,473 | Toughsheet Community Stadium | Darren Drysdale | Match Report | |
| 17 | Sat | 2025-11-22 | 15:00 | Stevenage | 0–0 | Doncaster Rovers | 4,015 | The Lamex Stadium | Elliot Bell | Match Report | |
| | | | | | | | | | | |
| 12 | Tue | 2025-11-25 | 19:45 | Luton Town | 2–1 | Huddersfield Town | 9,971 | Kenilworth Road Stadium | Andrew Humphries | Match Report | |
| 12 | Tue | 2025-11-25 | 19:45 | Peterborough United | 0–1 | Stevenage | 6,814 | Weston Homes Stadium | Edward Duckworth | Match Report | |
| | | | | | | | | | | |
| 18 | Sat | 2025-11-29 | 12:30 | Blackpool | 0–3 | Reading | 9,272 | Bloomfield Road Stadium | Thomas Parsons | Match Report | |
| 18 | Sat | 2025-11-29 | 12:30 | Stockport | 1–1 | Barnsley | 9,861 | Edgeley Park | Craig Hicks | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Huddersfield Town | 3–3 | AFC Wimbledon | 16,355 | Accu Stadium | Scott Oldham | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Luton Town | 1–1 | Bolton Wanderers | 11,204 | Kenilworth Road Stadium | Ruebyn Ricardo | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Rotherham United | 1–1 | Wycombe Wanderers | 8,360 | AESSEAL New York Stadium | Seb Stockbridge | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Burton Albion | 0–4 | Leyton Orient | 3,264 | Pirelli Stadium | Paul Howard | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Wigan Athletic | 0–0 | Stevenage | 9,842 | The Brick Community Stadium | Ross Joyce | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Bradford City | 1–0 | Exeter City | 19,713 | University of Bradford Stadium | Richard Eley | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Cardiff City | 3–0 | Mansfield Town | 18,174 | Cardiff City Stadium | Ben Atkinson | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Doncaster Rovers | 2–1 | Peterborough United | 9,654 | Eco-Power Stadium | Oliver Yates | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Lincoln City | 1–0 | Port Vale | 8,503 | LNER Stadium | Peter Wright | Match Report | |
| 18 | Sat | 2025-11-29 | 15:00 | Plymouth Argyle | 0–3 | Northampton Town | 15,419 | Home Park | Stephen Parkinson | Match Report | |
| | | | | | | | | | | |
| 19 | Tue | 2025-12-02 | 19:45 | Wigan Athletic | 1–0 | Burton Albion | 7,343 | The Brick Community Stadium | Martin Woods | Match Report | |
| | | | | | | | | | | |
| 16 | Sat | 2025-12-06 | 12:30 | Cardiff City | 3–2 | Huddersfield Town | 16,048 | Cardiff City Stadium | Elliot Bell | Match Report | |
| 16 | Sat | 2025-12-06 | 12:30 | Plymouth Argyle | 0–1 | Bradford City | 14,811 | Home Park | Thomas Parsons | Match Report | |
| | | | | | | | | | | |
| 19 | Tue | 2025-12-09 | 19:45 | Doncaster Rovers | 0–2 | Stockport | 7,929 | Eco-Power Stadium | Martin Woods | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Lincoln City | 3–1 | Barnsley | 7,768 | LNER Stadium | Dale Baines | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Leyton Orient | 1–1 | Luton Town | 3,176 | The BetWright Stadium | Darren Drysdale | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Mansfield Town | 0–1 | Bolton Wanderers | 7,628 | One Call Stadium | Thomas Parsons | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Wycombe Wanderers | 0–1 | Plymouth Argyle | 4,664 | Adams Park | Peter Wright | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Northampton Town | 1–1 | Huddersfield Town | 5,584 | Sixfields Stadium | James Durkin | Match Report | |
| 19 | Tue | 2025-12-09 | 19:45 | Exeter City | 1–0 | AFC Wimbledon | 5,579 | St James Park | Will Finnie | Match Report | |
| 19 | Tue | 2025-12-09 | 20:00 | Stevenage | 0–1 | Cardiff City | 4,428 | The Lamex Stadium | James Oldham | Match Report | |
| 19 | Tue | 2025-12-09 | 20:00 | Reading | 1–2 | Peterborough United | 9,183 | Select Car Leasing Stadium | Craig Hicks | Match Report | |
| 19 | Wed | 2025-12-10 | 19:45 | Rotherham United | 0–3 | Blackpool | 7,367 | AESSEAL New York Stadium | Neil Hair | Match Report | |
| | | | | | | | | | | |
| 20 | Sat | 2025-12-13 | 12:30 | Huddersfield Town | 1–1 | Wigan Athletic | 16,442 | Accu Stadium | Darren Drysdale | Match Report | |
| 20 | Sat | 2025-12-13 | 12:30 | AFC Wimbledon | 0–0 | Mansfield Town | 8,474 | The Cherry Red Records Stadium | Elliot Bell | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Luton Town | 2–2 | Port Vale | 10,867 | Kenilworth Road Stadium | Lee Swabey | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Bolton Wanderers | 2–1 | Exeter City | 20,622 | Toughsheet Community Stadium | Ben Speedie | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Peterborough United | 2–1 | Northampton Town | 5,260 | Weston Homes Stadium | John Busby | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Barnsley | 3–2 | Leyton Orient | 10,232 | Oakwell Stadium | Simon Mather | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Bradford City | 2–0 | Reading | 19,123 | University of Bradford Stadium | Paul Howard | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Stockport | 1–3 | Stevenage | 8,898 | Edgeley Park | Will Finnie | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Burton Albion | 0–0 | Wycombe Wanderers | 2,844 | Pirelli Stadium | Neil Hair | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Cardiff City | 4–3 | Doncaster Rovers | 18,934 | Cardiff City Stadium | Alex Chilowicz | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Plymouth Argyle | 1–0 | Rotherham United | 14,318 | Home Park | David Rock | Match Report | |
| 20 | Sat | 2025-12-13 | 15:00 | Blackpool | 2–2 | Lincoln City | 9,227 | Bloomfield Road Stadium | Seb Stockbridge | Match Report | |
| | | | | | | | | | | |
| 21 | Thu | 2025-12-18 | 20:00 | Reading | 3–2 | Luton Town | 9,252 | Select Car Leasing Stadium | Edward Duckworth | Match Report | |
| 21 | Fri | 2025-12-19 | 19:45 | Northampton Town | 3–1 | AFC Wimbledon | 6,252 | Sixfields Stadium | Carl Brook | Match Report | |
| 21 | Sat | 2025-12-20 | 12:30 | Wigan Athletic | 0–2 | Blackpool | 10,670 | The Brick Community Stadium | John Busby | Match Report | |
| 21 | Sat | 2025-12-20 | 12:30 | Stevenage | 2–2 | Burton Albion | 3,282 | The Lamex Stadium | Scott Oldham | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Doncaster Rovers | 1–5 | Plymouth Argyle | 8,370 | Eco-Power Stadium | Ross Joyce | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Lincoln City | 2–1 | Cardiff City | 9,913 | LNER Stadium | Richard Eley | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Exeter City | 3–0 | Barnsley | 6,471 | St James Park | Ben Atkinson | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Leyton Orient | 2–1 | Bradford City | 8,693 | The BetWright Stadium | Will Finnie | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Port Vale | 0–1 | Peterborough United | 7,533 | Vale Park | Kirsty Dowle | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Wycombe Wanderers | 2–1 | Bolton Wanderers | 6,197 | Adams Park | Craig Hicks | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Rotherham United | 1–3 | Huddersfield Town | 9,514 | AESSEAL New York Stadium | Matthew Russell | Match Report | |
| 21 | Sat | 2025-12-20 | 15:00 | Mansfield Town | 1–2 | Stockport | 8,086 | One Call Stadium | Alan Young | Match Report | |
| | | | | | | | | | | |
| 22 | Fri | 2025-12-26 | 15:00 | Peterborough United | 1–0 | Leyton Orient | 9,882 | Weston Homes Stadium | Abigail Byrne | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Barnsley | 2–3 | Mansfield Town | 12,856 | Oakwell Stadium | Martin Coy | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Cardiff City | 1–0 | Exeter City | 22,291 | Cardiff City Stadium | Tom Reeves | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Luton Town | 4–0 | Wycombe Wanderers | 11,596 | Kenilworth Road Stadium | Dean Whitestone | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Blackpool | 1–0 | Doncaster Rovers | 10,916 | Bloomfield Road Stadium | Matthew Corlett | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Plymouth Argyle | 1–4 | Reading | 16,434 | Home Park | James Durkin | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Bradford City | 2–1 | Wigan Athletic | 22,698 | University of Bradford Stadium | Seb Stockbridge | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Burton Albion | 5–1 | Northampton Town | 4,704 | Pirelli Stadium | Sam Mulhall | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Bolton Wanderers | 2–1 | Rotherham United | 23,416 | Toughsheet Community Stadium | Elliot Bell | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | AFC Wimbledon | 0–0 | Stevenage | 8,465 | The Cherry Red Records Stadium | David Rock | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Huddersfield Town | 5–0 | Port Vale | 1,862 | Accu Stadium | Martin Woods | Match Report | |
| 22 | Fri | 2025-12-26 | 15:00 | Stockport | 1–2 | Lincoln City | 9,690 | Edgeley Park | Paul Marsden | Match Report | |
| | | | | | | | | | | |
| 23 | Mon | 2025-12-29 | 19:45 | Stockport | 4–2 | Doncaster Rovers | 9,629 | Edgeley Park | Thomas Parsons | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Peterborough United | 1–1 | Reading | 7,225 | Weston Homes Stadium | Alex Chilowicz | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Bolton Wanderers | 0–1 | Mansfield Town | 22,320 | Toughsheet Community Stadium | Peter Wright | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | AFC Wimbledon | 0–1 | Exeter City | 8,621 | The Cherry Red Records Stadium | Jacob Miles | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Bradford City | 1–0 | Port Vale | 19,697 | University of Bradford Stadium | Scott Oldham | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Luton Town | 3–0 | Leyton Orient | 11,736 | Kenilworth Road Stadium | Neil Hair | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Barnsley | 0–2 | Lincoln City | 10,711 | Oakwell Stadium | Paul Howard | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Cardiff City | 2–1 | Stevenage | 19,678 | Cardiff City Stadium | Charles Breakspear | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Huddersfield Town | 2–0 | Northampton Town | 16,639 | Accu Stadium | Will Finnie | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Burton Albion | 0–2 | Wigan Athletic | 3,696 | Pirelli Stadium | Richie Watkins | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Blackpool | 4–0 | Rotherham United | 9,636 | Bloomfield Road Stadium | Oliver Yates | Match Report | |
| 23 | Mon | 2025-12-29 | 19:45 | Plymouth Argyle | 1–1 | Wycombe Wanderers | 15,766 | Home Park | Carl Brook | Match Report | |
| | | | | | | | | | | |
| 24 | Thu | 2026-01-01 | 15:00 | Rotherham United | 0–2 | Peterborough United | 8,429 | AESSEAL New York Stadium | Matthew Corlett | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Northampton Town | 0–0 | Stockport | 7,050 | Sixfields Stadium | Darren Drysdale | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Mansfield Town | 3–0 | Bradford City | 9,077 | One Call Stadium | Ross Joyce | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Lincoln City | 1–1 | Huddersfield Town | 10,110 | LNER Stadium | Jamie O'Connor | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Leyton Orient | 1–3 | AFC Wimbledon | 8,643 | The BetWright Stadium | Ben Atkinson | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Wigan Athletic | 1–1 | Barnsley | 9,079 | The Brick Community Stadium | Ben Speedie | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Exeter City | 1–0 | Luton Town | 7,912 | St James Park | Matthew Russell | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Wycombe Wanderers | 1–1 | Cardiff City | 7,364 | Adams Park | Alan Young | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Reading | 2–0 | Burton Albion | 12,104 | Select Car Leasing Stadium | Tom Reeves | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Port Vale | 5–1 | Blackpool | 7,541 | Vale Park | Edward Duckworth | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Stevenage | 1–1 | Plymouth Argyle | 5,042 | The Lamex Stadium | Richard Eley | Match Report | |
| 24 | Thu | 2026-01-01 | 15:00 | Doncaster Rovers | 1–1 | Bolton Wanderers | 10,456 | Eco-Power Stadium | Martin Coy | Match Report | |
| | | | | | | | | | | |
| 25 | Sun | 2026-01-04 | 12:00 | Lincoln City | 5–2 | Peterborough United | 9,833 | LNER Stadium | Charles Breakspear | Match Report | |
| 25 | Sun | 2026-01-04 | 12:00 | Bolton Wanderers | 0–0 | Northampton Town | 20,333 | Toughsheet Community Stadium | Alex Chilowicz | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Wycombe Wanderers | 2–0 | AFC Wimbledon | 5,856 | Adams Park | Thomas Parsons | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Cardiff City | 1–0 | Wigan Athletic | 18,174 | Cardiff City Stadium | Will Finnie | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Blackpool | 1–2 | Bradford City | 10,894 | Bloomfield Road Stadium | Simon Mather | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Reading | 1–0 | Stockport | 11,703 | Select Car Leasing Stadium | Jacob Miles | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Huddersfield Town | 2–2 | Exeter City | 16,071 | Accu Stadium | Abigail Byrne | Match Report | |
| 25 | Sun | 2026-01-04 | 15:00 | Plymouth Argyle | 3–0 | Burton Albion | 14,662 | Home Park | Oliver Yates | Match Report | |
| | | | | | | | | | | |
| 26 | Sat | 2026-01-10 | 12:30 | Luton Town | 2–1 | Stevenage | 11,434 | Kenilworth Road Stadium | Simon Mather | Match Report | |
| 26 | Sat | 2026-01-10 | 12:30 | Stockport | 1–0 | Huddersfield Town | 9,486 | Edgeley Park | David Rock | Match Report | |
| 26 | Sat | 2026-01-10 | 15:00 | Peterborough United | 3–1 | Bolton Wanderers | 7,838 | Weston Homes Stadium | Oliver Yates | Match Report | |
| 26 | Sat | 2026-01-10 | 15:00 | Leyton Orient | 1–1 | Cardiff City | 8,423 | The BetWright Stadium | Scott Oldham | Match Report | |
| | | | | | | | | | | |
| 27 | Sat | 2026-01-17 | 12:30 | Bradford City | 1–2 | Cardiff City | 21,571 | University of Bradford Stadium | David Rock | Match Report | |
| 27 | Sat | 2026-01-17 | 12:30 | Wigan Athletic | 0–1 | Bolton Wanderers | 15,112 | The Brick Community Stadium | Stephen Martin | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Barnsley | 2–1 | Blackpool | 10,266 | Oakwell Stadium | Andrew Humphries | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Peterborough United | 0–1 | Plymouth Argyle | 8,634 | Weston Homes Stadium | Ben Atkinson | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | AFC Wimbledon | 0–1 | Doncaster Rovers | 8,092 | The Cherry Red Records Stadium | James Durkin | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Leyton Orient | 3–1 | Reading | 8,492 | The BetWright Stadium | Elliot Bell | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Exeter City | 3–0 | Stevenage | 6,302 | St James Park | Martin Woods | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Mansfield Town | 3–0 | Port Vale | 8,364 | One Call Stadium | Abigail Byrne | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Stockport | 3–2 | Rotherham United | 9,141 | Edgeley Park | Aaron Bannister | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Northampton Town | 1–2 | Wycombe Wanderers | 6,758 | Sixfields Stadium | Lee Swabey | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Luton Town | 2–2 | Lincoln City | 11,230 | Kenilworth Road Stadium | Scott Oldham | Match Report | |
| 27 | Sat | 2026-01-17 | 15:00 | Burton Albion | 3–1 | Huddersfield Town | 4,318 | Pirelli Stadium | Simon Mather | Match Report | |
| | | | | | | | | | | |
| 16 | Tue | 2026-01-20 | 19:45 | Stevenage | 0–0 | Bolton Wanderers | 4,786 | The Lamex Stadium | Stephen Parkinson | Match Report | |
| | | | | | | | | | | |
| 28 | Thu | 2026-01-22 | 20:00 | Lincoln City | 2–1 | Burton Albion | 9,514 | LNER Stadium | Matthew Corlett | Match Report | |
| 28 | Sat | 2026-01-24 | 12:30 | Huddersfield Town | 1–0 | Bradford City | 21,422 | Accu Stadium | Edward Duckworth | Match Report | |
| 28 | Sat | 2026-01-24 | 12:30 | Plymouth Argyle | 1–0 | Luton Town | 15,949 | Home Park | Elliot Bell | Match Report | |
| 28 | Sat | 2026-01-24 | 12:30 | Cardiff City | 1–1 | Stockport | 19,684 | Cardiff City Stadium | Paul Howard | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Stevenage | 1–1 | Mansfield Town | 4,041 | The Lamex Stadium | Ben Atkinson | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Bolton Wanderers | 2–1 | Leyton Orient | 20,352 | Toughsheet Community Stadium | Seb Stockbridge | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Rotherham United | 1–1 | AFC Wimbledon | 7,787 | AESSEAL New York Stadium | Richard Eley | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Reading | 2–2 | Barnsley | 12,101 | Select Car Leasing Stadium | Leigh Doughty | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Doncaster Rovers | 3–3 | Wigan Athletic | 8,128 | Eco-Power Stadium | David Rock | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Blackpool | 2–0 | Northampton Town | 10,009 | Bloomfield Road Stadium | Peter Wright | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Wycombe Wanderers | 0–2 | Peterborough United | 7,166 | Adams Park | Tom Reeves | Match Report | |
| 28 | Sat | 2026-01-24 | 15:00 | Port Vale | 1–3 | Exeter City | 8,432 | Vale Park | Andrew Humphries | Match Report | |
| | | | | | | | | | | |
| 29 | Tue | 2026-01-27 | 19:45 | Lincoln City | 3–0 | Bradford City | 10,064 | LNER Stadium | Stephen Parkinson | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Huddersfield Town | 1–0 | Luton Town | 15,367 | Accu Stadium | Matthew Corlett | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Blackpool | 1–2 | Stockport | 9,708 | Bloomfield Road Stadium | Ben Speedie | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Rotherham United | 2–1 | Northampton Town | 7,228 | AESSEAL New York Stadium | Alan Young | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Bolton Wanderers | 2–1 | Burton Albion | 18,820 | Toughsheet Community Stadium | Thomas Parsons | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Stevenage | 1–0 | Peterborough United | 4,249 | The Lamex Stadium | Andrew Humphries | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Wycombe Wanderers | 2–0 | Wigan Athletic | 4,190 | Adams Park | Paul Howard | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Cardiff City | 4–0 | Barnsley | 17,841 | Cardiff City Stadium | Lee Swabey | Match Report | |
| 29 | Tue | 2026-01-27 | 19:45 | Plymouth Argyle | 1–1 | Mansfield Town | 14,131 | Home Park | Will Finnie | Match Report | |
| 29 | Tue | 2026-01-27 | 20:00 | Reading | 2–2 | Exeter City | 8,404 | Select Car Leasing Stadium | Charles Breakspear | Match Report | |
| 29 | Tue | 2026-01-27 | 20:00 | Doncaster Rovers | 3–0 | Leyton Orient | 7,033 | Eco-Power Stadium | Martin Woods | Match Report | |
| | | | | | | | | | | |
| 30 | Sat | 2026-01-31 | 12:30 | Bradford City | 1–0 | Doncaster Rovers | 19,775 | University of Bradford Stadium | Craig Hicks | Match Report | |
| 30 | Sat | 2026-01-31 | 12:30 | AFC Wimbledon | 0–1 | Bolton Wanderers | 8,659 | The Cherry Red Records Stadium | Andrew Humphries | Match Report | |
| 30 | Sat | 2026-01-31 | 14:00 | Northampton Town | 0–2 | Reading | 7,038 | Sixfields Stadium | Jamie O'Connor | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Mansfield Town | 0–0 | Wycombe Wanderers | 7,401 | One Call Stadium | Ben Speedie | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Luton Town | 1–0 | Blackpool | 10,697 | Kenilworth Road Stadium | Martin Woods | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Stockport | 2–1 | Plymouth Argyle | 10,117 | Edgeley Park | Samuel Allison | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Leyton Orient | 0–1 | Port Vale | 7,692 | The BetWright Stadium | David Rock | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Exeter City | 0–4 | Rotherham United | 7,171 | St James Park | Paul Howard | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Barnsley | 3–1 | Stevenage | 9,823 | Oakwell Stadium | Seb Stockbridge | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Wigan Athletic | 0–1 | Lincoln City | 9,436 | The Brick Community Stadium | Carl Brook | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Peterborough United | 2–3 | Huddersfield Town | 9,261 | Weston Homes Stadium | Lee Swabey | Match Report | |
| 30 | Sat | 2026-01-31 | 15:00 | Burton Albion | 2–2 | Cardiff City | 4,864 | Pirelli Stadium | Martin Coy | Match Report | |
| | | | | | | | | | | |
| 29 | Tue | 2026-02-03 | 19:00 | Port Vale | 0–1 | AFC Wimbledon | 4,534 | Vale Park | James Oldham | Match Report | |
| | | | | | | | | | | |
| 16 | Tue | 2026-02-03 | 19:45 | Barnsley | 2–2 | Northampton Town | 8,806 | Oakwell Stadium | Thomas Parsons | Match Report | |
| | | | | | | | | | | |
| 26 | Tue | 2026-02-03 | 19:45 | Burton Albion | 1–2 | Doncaster Rovers | 3,069 | Pirelli Stadium | Charles Breakspear | Match Report | |
| | | | | | | | | | | |
| 31 | Sat | 2026-02-07 | 12:31 | Port Vale | 2–2 | Burton Albion | 6,232 | Vale Park | Will Finnie | Match Report | |
| 31 | Sat | 2026-02-07 | 12:31 | Luton Town | 2–1 | Bradford City | 11,000 | Kenilworth Road Stadium | Richard Eley | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Plymouth Argyle | 1–4 | Lincoln City | 15,547 | Home Park | Stephen Martin | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Stockport | 0–0 | Leyton Orient | 9,162 | Edgeley Park | Andrew Humphries | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Northampton Town | 3–1 | Stevenage | 6,344 | Sixfields Stadium | Oliver Yates | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Mansfield Town | 0–0 | Exeter City | 7,579 | One Call Stadium | Simon Mather | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Peterborough United | 6–1 | Wigan Athletic | 8,443 | Weston Homes Stadium | Stephen Parkinson | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | AFC Wimbledon | 3–2 | Reading | 8,574 | The Cherry Red Records Stadium | Dale Baines | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Huddersfield Town | 2–2 | Blackpool | 16,951 | Accu Stadium | Ross Joyce | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Wycombe Wanderers | 4–0 | Doncaster Rovers | 5,063 | Adams Park | Kirsty Dowle | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Bolton Wanderers | 3–2 | Barnsley | 21,415 | Toughsheet Community Stadium | Scott Oldham | Match Report | |
| 31 | Sat | 2026-02-07 | 15:01 | Rotherham United | 0–3 | Cardiff City | 8,786 | AESSEAL New York Stadium | Seb Stockbridge | Match Report | |
| | | | | | | | | | | |
| 16 | Tue | 2026-02-10 | 19:45 | Mansfield Town | 1–2 | Peterborough United | 7,167 | One Call Stadium | Carl Brook | Match Report | |
| 16 | Tue | 2026-02-10 | 19:45 | Wigan Athletic | 1–2 | Reading | 7,973 | The Brick Community Stadium | Lee Swabey | Match Report | |
| | | | | | | | | | | |
| 32 | Sat | 2026-02-14 | 12:30 | Reading | 3–2 | Wycombe Wanderers | 13,105 | Select Car Leasing Stadium | Martin Woods | Match Report | |
| 32 | Sat | 2026-02-14 | 12:30 | Stevenage | 1–0 | Huddersfield Town | 3,436 | The Lamex Stadium | Neil Hair | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Cardiff City | 3–1 | Luton Town | 19,524 | Cardiff City Stadium | Oliver Yates | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Exeter City | 0–0 | Northampton Town | 6,310 | St James Park | Oliver Mackey | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Lincoln City | 1–1 | Bolton Wanderers | 10,235 | LNER Stadium | Will Finnie | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Barnsley | 3–3 | AFC Wimbledon | 9,640 | Oakwell Stadium | Charles Breakspear | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Bradford City | 2–0 | Peterborough United | 19,389 | University of Bradford Stadium | Samuel Allison | Match Report | |
| 32 | Sat | 2026-02-14 | 15:00 | Blackpool | 0–4 | Plymouth Argyle | 9,858 | Bloomfield Road Stadium | Paul Howard | Match Report | |
| | | | | | | | | | | |
| 33 | Tue | 2026-02-17 | 19:45 | Doncaster Rovers | 1–0 | Huddersfield Town | 9,118 | Eco-Power Stadium | Seb Stockbridge | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Lincoln City | 4–0 | Northampton Town | 9,242 | LNER Stadium | Kirsty Dowle | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Barnsley | 2–1 | Peterborough United | 9,257 | Oakwell Stadium | Zac Kennard-Kettle | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Cardiff City | 4–1 | AFC Wimbledon | 18,028 | Cardiff City Stadium | Simon Mather | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Leyton Orient | 1–3 | Plymouth Argyle | 3,585 | The BetWright Stadium | Alex Chilowicz | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Exeter City | 1–1 | Wycombe Wanderers | 6,188 | St James Park | Gavin Ward | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Bradford City | 1–0 | Stockport | 22,488 | University of Bradford Stadium | Ross Joyce | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Burton Albion | 1–0 | Rotherham United | 3,517 | Pirelli Stadium | Abigail Byrne | Match Report | |
| 33 | Tue | 2026-02-17 | 19:45 | Stevenage | 2–1 | Port Vale | 3,074 | The Lamex Stadium | James Durkin | Match Report | |
| 33 | Tue | 2026-02-17 | 20:00 | Blackpool | 1–0 | Mansfield Town | 8,905 | Bloomfield Road Stadium | Andrew Kitchen | Match Report | |
| 33 | Tue | 2026-02-17 | 20:00 | Reading | 1–1 | Bolton Wanderers | 10,025 | Select Car Leasing Stadium | Peter Wright | Match Report | |
| 33 | Wed | 2026-02-18 | 19:45 | Wigan Athletic | 1–0 | Luton Town | 8,440 | The Brick Community Stadium | Jamie O'Connor | Match Report | |
| | | | | | | | | | | |
| 34 | Sat | 2026-02-21 | 12:30 | Wycombe Wanderers | 3–1 | Stevenage | 4,727 | Adams Park | Matthew Russell | Match Report | |
| 34 | Sat | 2026-02-21 | 12:30 | Rotherham United | 1–2 | Doncaster Rovers | 10,049 | AESSEAL New York Stadium | Simon Mather | Match Report | |
| 34 | Sat | 2026-02-21 | 12:30 | Plymouth Argyle | 5–2 | Cardiff City | 16,713 | Home Park | Darren Drysdale | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Peterborough United | 3–3 | Exeter City | 7,341 | Weston Homes Stadium | Elliott Swallow | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Bolton Wanderers | 2–2 | Blackpool | 23,172 | Toughsheet Community Stadium | Richard Eley | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Luton Town | 1–1 | Burton Albion | 10,364 | Kenilworth Road Stadium | Seb Stockbridge | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Port Vale | 1–1 | Reading | 7,023 | Vale Park | Carl Brook | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Huddersfield Town | 2–1 | Barnsley | 18,187 | Accu Stadium | Peter Wright | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Northampton Town | 1–2 | Leyton Orient | 6,441 | Sixfields Stadium | Edward Duckworth | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Stockport | 4–2 | Wigan Athletic | 9,928 | Edgeley Park | Matthew Corlett | Match Report | |
| 34 | Sat | 2026-02-21 | 15:00 | Mansfield Town | 0–2 | Lincoln City | 9,050 | One Call Stadium | David Rock | Match Report | |
| 34 | Sat | 2026-02-21 | 15:30 | AFC Wimbledon | 3–1 | Bradford City | 8,486 | The Cherry Red Records Stadium | Ross Martin | Match Report | |
| | | | | | | | | | | |
| 26 | Tue | 2026-02-24 | 19:45 | Northampton Town | 0–1 | Port Vale | 5,335 | Sixfields Stadium | Charles Breakspear | Match Report | |
| 26 | Tue | 2026-02-24 | 19:45 | Bradford City | 1–0 | Rotherham United | 18,187 | University of Bradford Stadium | Martin Woods | Match Report | |
| | | | | | | | | | | |
| 32 | Tue | 2026-02-24 | 19:45 | Burton Albion | 3–0 | Stockport | 3,224 | Pirelli Stadium | Stephen Parkinson | Match Report | |
| | | | | | | | | | | |
| 35 | Sat | 2026-02-28 | 12:30 | Northampton Town | 1–1 | Peterborough United | 7,637 | Sixfields Stadium | David Rock | Match Report | |
| 35 | Sat | 2026-02-28 | 12:30 | Leyton Orient | 1–3 | Barnsley | 8,313 | The BetWright Stadium | Carl Brook | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Exeter City | 1–5 | Bolton Wanderers | 7,341 | St James Park | Craig Hicks | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Lincoln City | 4–0 | Blackpool | 9,812 | LNER Stadium | Robert Madley | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Reading | 2–1 | Bradford City | 13,106 | Select Car Leasing Stadium | Ben Speedie | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Doncaster Rovers | 0–4 | Cardiff City | 11,618 | Eco-Power Stadium | Edward Duckworth | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Mansfield Town | 2–2 | AFC Wimbledon | 7,693 | One Call Stadium | Seb Stockbridge | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Stevenage | 2–1 | Stockport | 3,974 | The Lamex Stadium | Jamie O'Connor | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Port Vale | 1–1 | Luton Town | 7,335 | Vale Park | Lee Swabey | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Rotherham United | 1–0 | Plymouth Argyle | 8,502 | AESSEAL New York Stadium | Zac Kennard-Kettle | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Wigan Athletic | 1–0 | Huddersfield Town | 12,811 | The Brick Community Stadium | Charles Breakspear | Match Report | |
| 35 | Sat | 2026-02-28 | 15:00 | Wycombe Wanderers | 3–0 | Burton Albion | 4,797 | Adams Park | Alex Chilowicz | Match Report | |
| | | | | | | | | | | |
| 17 | Tue | 2026-03-03 | 19:45 | Exeter City | 1–1 | Burton Albion | 5,221 | St James Park | Paul Howard | Match Report | |
| | | | | | | | | | | |
| 25 | Tue | 2026-03-03 | 19:45 | Rotherham United | 0–0 | Mansfield Town | 9,243 | AESSEAL New York Stadium | Scott Oldham | Match Report | |
| | | | | | | | | | | |
| 26 | Tue | 2026-03-03 | 19:45 | Barnsley | 0–1 | Wycombe Wanderers | 8,608 | Oakwell Stadium | Oliver Yates | Match Report | |
| | | | | | | | | | | |
| 36 | Sat | 2026-03-07 | 12:30 | Huddersfield Town | 1–0 | Rotherham United | 16,592 | Accu Stadium | Martin Coy | Match Report | |
| 36 | Sat | 2026-03-07 | 12:30 | Cardiff City | 0–2 | Lincoln City | 27,280 | Cardiff City Stadium | Tom Reeves | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Blackpool | 1–1 | Wigan Athletic | 11,210 | Bloomfield Road Stadium | Matthew Russell | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Bradford City | 2–1 | Leyton Orient | 20,123 | University of Bradford Stadium | Oliver Yates | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Burton Albion | 0–1 | Stevenage | 3,198 | Pirelli Stadium | Craig Hicks | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Luton Town | 2–3 | Reading | 11,374 | Kenilworth Road Stadium | Oliver Mackey | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Barnsley | 2–1 | Exeter City | 9,706 | Oakwell Stadium | Ross Joyce | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Bolton Wanderers | 3–2 | Wycombe Wanderers | 20,512 | Toughsheet Community Stadium | Matthew Corlett | Match Report | |
| 36 | Sat | 2026-03-07 | 15:00 | Plymouth Argyle | 2–1 | Doncaster Rovers | 16,046 | Home Park | Stephen Parkinson | Match Report | |
| 36 | Sun | 2026-03-08 | 12:00 | AFC Wimbledon | 1–0 | Northampton Town | 8,142 | The Cherry Red Records Stadium | Elliot Bell | Match Report | |
| | | | | | | | | | | |
| 12 | Tue | 2026-03-10 | 19:45 | Barnsley | 1–1 | Cardiff City | 9,677 | Oakwell Stadium | Will Finnie | Match Report | |
| | | | | | | | | | | |
| 25 | Tue | 2026-03-10 | 19:45 | Doncaster Rovers | 1–1 | Luton Town | 7,470 | Eco-Power Stadium | Andrew Humphries | Match Report | |
| 25 | Tue | 2026-03-10 | 19:45 | Stevenage | 1–2 | Leyton Orient | 3,559 | The Lamex Stadium | Charles Breakspear | Match Report | |
| | | | | | | | | | | |
| 26 | Tue | 2026-03-10 | 19:45 | Exeter City | 0–1 | Lincoln City | 5,142 | St James Park | Carl Brook | Match Report | |
| 26 | Tue | 2026-03-10 | 19:45 | Wigan Athletic | 0–3 | Plymouth Argyle | 8,055 | The Brick Community Stadium | Scott Oldham | Match Report | |
| 26 | Tue | 2026-03-10 | 19:45 | Mansfield Town | 1–0 | Reading | 7,218 | One Call Stadium | Thomas Parsons | Match Report | |
| | | | | | | | | | | |
| 19 | Wed | 2026-03-11 | 19:45 | Port Vale | 0–2 | Bradford City | 6,098 | Vale Park | Paul Howard | Match Report | |
| | | | | | | | | | | |
| 26 | Wed | 2026-03-11 | 19:45 | AFC Wimbledon | 4–1 | Blackpool | 7,535 | The Cherry Red Records Stadium | Lee Swabey | Match Report | |
| | | | | | | | | | | |
| 37 | Sat | 2026-03-14 | 12:30 | Lincoln City | 3–1 | Stockport | 9,759 | LNER Stadium | Charles Breakspear | Match Report | |
| 37 | Sat | 2026-03-14 | 12:30 | Rotherham United | 2–2 | Bolton Wanderers | 9,348 | AESSEAL New York Stadium | James Oldham | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Port Vale | 0–0 | Huddersfield Town | 6,878 | Vale Park | Thomas Parsons | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Doncaster Rovers | 2–1 | Blackpool | 8,662 | Eco-Power Stadium | Carl Brook | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Mansfield Town | 2–2 | Barnsley | 8,538 | One Call Stadium | Martin Woods | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Wigan Athletic | 2–0 | Bradford City | 16,710 | The Brick Community Stadium | Andrew Humphries | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Leyton Orient | 2–1 | Peterborough United | 8,514 | The BetWright Stadium | Robert Madley | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Wycombe Wanderers | 1–2 | Luton Town | 6,761 | Adams Park | Craig Hicks | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Northampton Town | 0–2 | Burton Albion | 6,408 | Sixfields Stadium | Lee Swabey | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Exeter City | 0–4 | Cardiff City | 7,180 | St James Park | Ben Speedie | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Stevenage | 1–0 | AFC Wimbledon | 4,089 | The Lamex Stadium | Declan Bourne | Match Report | |
| 37 | Sat | 2026-03-14 | 15:00 | Reading | 2–2 | Plymouth Argyle | 16,277 | Select Car Leasing Stadium | Seb Stockbridge | Match Report | |
| | | | | | | | | | | |
| 38 | Tue | 2026-03-17 | 19:45 | Cardiff City | 0–2 | Wycombe Wanderers | 16,663 | Cardiff City Stadium | Carl Brook | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Luton Town | 3–2 | Exeter City | 9,216 | Kenilworth Road Stadium | Alex Chilowicz | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Bradford City | 1–1 | Mansfield Town | 19,716 | University of Bradford Stadium | James Oldham | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Burton Albion | 1–2 | Reading | 2,902 | Pirelli Stadium | Edward Duckworth | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | AFC Wimbledon | 2–4 | Leyton Orient | 8,275 | The Cherry Red Records Stadium | Alan Dale | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Peterborough United | 5–0 | Rotherham United | 6,112 | Weston Homes Stadium | Matthew Russell | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Huddersfield Town | 2–2 | Lincoln City | 16,301 | Accu Stadium | Ben Speedie | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Plymouth Argyle | 1–0 | Stevenage | 13,945 | Home Park | Elliot Bell | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Bolton Wanderers | 0–0 | Doncaster Rovers | 19,570 | Toughsheet Community Stadium | Jamie O'Connor | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Stockport | 2–1 | Northampton Town | 8,085 | Edgeley Park | Scott Oldham | Match Report | |
| 38 | Tue | 2026-03-17 | 19:45 | Blackpool | 3–2 | Port Vale | 8,567 | Bloomfield Road Stadium | Will Finnie | Match Report | |
| 38 | Tue | 2026-03-17 | 20:00 | Barnsley | 1–1 | Wigan Athletic | 9,871 | Oakwell Stadium | Ross Martin | Match Report | |
| | | | | | | | | | | |
| 39 | Sat | 2026-03-21 | 12:30 | Cardiff City | 0–0 | Blackpool | 18,026 | Cardiff City Stadium | Stephen Parkinson | Match Report | |
| 39 | Sat | 2026-03-21 | 12:30 | Luton Town | 1–1 | Stockport | 10,791 | Kenilworth Road Stadium | Robert Madley | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Leyton Orient | 2–0 | Wycombe Wanderers | 8,697 | The BetWright Stadium | Simon Mather | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Plymouth Argyle | 3–1 | Huddersfield Town | 16,266 | Home Park | Ruebyn Ricardo | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Mansfield Town | 4–1 | Northampton Town | 7,633 | One Call Stadium | Matthew Corlett | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | AFC Wimbledon | 1–1 | Peterborough United | 8,460 | The Cherry Red Records Stadium | Scott Jackson | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Burton Albion | 2–1 | Bradford City | 4,522 | Pirelli Stadium | Carl Brook | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Wigan Athletic | 2–0 | Exeter City | 9,506 | The Brick Community Stadium | Edward Duckworth | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Port Vale | 1–0 | Bolton Wanderers | 8,442 | Vale Park | Tom Reeves | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Lincoln City | 3–0 | Rotherham United | 10,071 | LNER Stadium | Craig Hicks | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Barnsley | 0–1 | Doncaster Rovers | 13,026 | Oakwell Stadium | David Rock | Match Report | |
| 39 | Sat | 2026-03-21 | 15:00 | Stevenage | 1–0 | Reading | 4,502 | The Lamex Stadium | Scott Oldham | Match Report | |
| | | | | | | | | | | |
| 32 | Tue | 2026-03-24 | 19:45 | Doncaster Rovers | 1–0 | Port Vale | 7,178 | Eco-Power Stadium | Michael Barlow | Match Report | |
| | | | | | | | | | | |
| 40 | Sat | 2026-03-28 | 15:00 | Reading | 3–0 | Wigan Athletic | 11,981 | Select Car Leasing Stadium | Steven Copeland | Match Report | |
| 40 | Sat | 2026-03-28 | 15:00 | Exeter City | 0–0 | Leyton Orient | 6,772 | St James Park | Tim Robinson | Match Report | |
| 40 | Sat | 2026-03-28 | 15:00 | Blackpool | 1–0 | Burton Albion | 11,060 | Bloomfield Road Stadium | Martin Coy | Match Report | |
| 40 | Sat | 2026-03-28 | 15:00 | Wycombe Wanderers | 4–0 | Port Vale | 5,073 | Adams Park | Darren Drysdale | Match Report | |
| 40 | Sat | 2026-03-28 | 15:00 | Stockport | 3–0 | AFC Wimbledon | 9,540 | Edgeley Park | Craig Hicks | Match Report | |
| | | | | | | | | | | |
| 41 | Thu | 2026-04-02 | 19:45 | Wigan Athletic | 0–0 | Leyton Orient | 9,239 | The Brick Community Stadium | Tom Reeves | Match Report | |
| 41 | Fri | 2026-04-03 | 12:30 | Doncaster Rovers | 0–2 | Mansfield Town | 10,887 | Eco-Power Stadium | Lee Swabey | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Bradford City | 1–0 | Northampton Town | 19,105 | University of Bradford Stadium | Martin Coy | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Rotherham United | 0–0 | Stevenage | 7,920 | AESSEAL New York Stadium | Carl Brook | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Lincoln City | 1–0 | AFC Wimbledon | 10,088 | LNER Stadium | Edward Duckworth | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Luton Town | 2–1 | Peterborough United | 11,429 | Kenilworth Road Stadium | Isaac Searle | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Stockport | 3–0 | Wycombe Wanderers | 9,476 | Edgeley Park | Elliot Bell | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Burton Albion | 1–1 | Barnsley | 4,203 | Pirelli Stadium | Kirsty Dowle | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Huddersfield Town | 1–1 | Reading | 17,148 | Accu Stadium | Will Finnie | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Blackpool | 1–0 | Exeter City | 10,950 | Bloomfield Road Stadium | Neil Hair | Match Report | |
| 41 | Fri | 2026-04-03 | 15:00 | Plymouth Argyle | 1–2 | Bolton Wanderers | 16,616 | Home Park | Charles Breakspear | Match Report | |
| | | | | | | | | | | |
| 42 | Mon | 2026-04-06 | 15:00 | Mansfield Town | 0–0 | Burton Albion | 8,049 | One Call Stadium | Matthew Russell | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Wycombe Wanderers | 1–2 | Bradford City | 6,718 | Adams Park | Will Finnie | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | AFC Wimbledon | 0–3 | Luton Town | 8,668 | The Cherry Red Records Stadium | Carl Brook | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Northampton Town | 1–3 | Wigan Athletic | 6,148 | Sixfields Stadium | Alex Chilowicz | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Stevenage | 1–0 | Blackpool | 4,348 | The Lamex Stadium | David Rock | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Exeter City | 3–0 | Doncaster Rovers | 6,638 | St James Park | Alan Dale | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Peterborough United | 1–1 | Cardiff City | 9,350 | Weston Homes Stadium | Thomas Parsons | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Bolton Wanderers | 2–2 | Stockport | 23,855 | Toughsheet Community Stadium | Stephen Martin | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Reading | 1–2 | Lincoln City | 17,144 | Select Car Leasing Stadium | Simon Mather | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Leyton Orient | 1–2 | Huddersfield Town | 8,697 | The BetWright Stadium | Matthew Corlett | Match Report | |
| 42 | Mon | 2026-04-06 | 15:00 | Barnsley | 0–3 | Plymouth Argyle | 10,722 | Oakwell Stadium | Tom Reeves | Match Report | |
| 42 | Tue | 2026-04-07 | 19:45 | Port Vale | 1–0 | Rotherham United | 4,523 | Vale Park | Ross Joyce | Match Report | |
| | | | | | | | | | | |
| 43 | Sat | 2026-04-11 | 12:30 | Cardiff City | 2–0 | Bolton Wanderers | 21,640 | Cardiff City Stadium | Elliot Bell | Match Report | |
| 43 | Sat | 2026-04-11 | 12:30 | Lincoln City | 2–1 | Leyton Orient | 9,919 | LNER Stadium | Ross Joyce | Match Report | |
| 43 | Sat | 2026-04-11 | 12:30 | Plymouth Argyle | 2–2 | Exeter City | 17,016 | Home Park | Matthew Corlett | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Doncaster Rovers | 1–0 | Reading | 8,904 | Eco-Power Stadium | Scott Jackson | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Wigan Athletic | 2–1 | Mansfield Town | 10,196 | The Brick Community Stadium | Oliver Yates | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Burton Albion | 1–0 | AFC Wimbledon | 3,654 | Pirelli Stadium | Martin Woods | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Blackpool | 3–1 | Peterborough United | 9,555 | Bloomfield Road Stadium | Charles Breakspear | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Rotherham United | 1–3 | Barnsley | 9,575 | AESSEAL New York Stadium | Lee Swabey | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Bradford City | 0–1 | Stevenage | 19,367 | University of Bradford Stadium | Tom Reeves | Match Report | |
| 43 | Sat | 2026-04-11 | 15:00 | Huddersfield Town | 3–3 | Wycombe Wanderers | 16,477 | Accu Stadium | James Oldham | Match Report | |
| | | | | | | | | | | |
| 25 | Tue | 2026-04-14 | 19:45 | Port Vale | 0–0 | Barnsley | 4,863 | Vale Park | Oliver Mackey | Match Report | |
| | | | | | | | | | | |
| 32 | Tue | 2026-04-14 | 19:45 | Leyton Orient | 0–0 | Mansfield Town | 7,941 | The BetWright Stadium | Ross Martin | Match Report | |
| 32 | Tue | 2026-04-14 | 19:45 | Wigan Athletic | 3–0 | Rotherham United | 7,989 | The Brick Community Stadium | Ben Speedie | Match Report | |
| | | | | | | | | | | |
| 40 | Tue | 2026-04-14 | 19:45 | Huddersfield Town | 1–1 | Cardiff City | 16,325 | Accu Stadium | Andrew Humphries | Match Report | |
| 40 | Tue | 2026-04-14 | 19:45 | Bolton Wanderers | 5–1 | Stevenage | 19,177 | Toughsheet Community Stadium | Thomas Parsons | Match Report | |
| | | | | | | | | | | |
| 16 | Wed | 2026-04-15 | 19:45 | AFC Wimbledon | 0–2 | Stockport | 8,040 | The Cherry Red Records Stadium | Darren Drysdale | Match Report | |
| | | | | | | | | | | |
| 43 | Wed | 2026-04-15 | 19:45 | Luton Town | 2–1 | Northampton Town | 9,638 | Kenilworth Road Stadium | James Durkin | Match Report | |
| | | | | | | | | | | |
| 36 | Thu | 2026-04-16 | 19:45 | Peterborough United | 1–3 | Port Vale | 6,533 | Weston Homes Stadium | Harry Wager | Match Report | |
| | | | | | | | | | | |
| 44 | Sat | 2026-04-18 | 12:30 | Bolton Wanderers | 3–3 | Huddersfield Town | 21,891 | Toughsheet Community Stadium | Jacob Miles | Match Report | |
| 44 | Sat | 2026-04-18 | 12:30 | Barnsley | 2–2 | Bradford City | 14,906 | Oakwell Stadium | Charles Breakspear | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Wycombe Wanderers | 0–1 | Blackpool | 5,797 | Adams Park | Andrew Humphries | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Northampton Town | 1–3 | Doncaster Rovers | 6,434 | Sixfields Stadium | Dale Baines | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Leyton Orient | 0–2 | Rotherham United | 7,817 | The BetWright Stadium | James Bell | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | AFC Wimbledon | 1–3 | Plymouth Argyle | 8,736 | The Cherry Red Records Stadium | Elliott Swallow | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Reading | 1–3 | Cardiff City | 13,938 | Select Car Leasing Stadium | Matthew Corlett | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Mansfield Town | 2–2 | Luton Town | 6,418 | One Call Stadium | Edward Duckworth | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Stevenage | 2–2 | Lincoln City | 5,647 | The Lamex Stadium | Neil Hair | Match Report | |
| 44 | Sat | 2026-04-18 | 15:00 | Exeter City | 3–3 | Stockport | 6,653 | St James Park | Kirsty Dowle | Match Report | |
| 44 | Sun | 2026-04-19 | 13:00 | Peterborough United | 1–1 | Burton Albion | 7,731 | Weston Homes Stadium | Steven Copeland | Match Report | |
| 44 | Sun | 2026-04-19 | 15:00 | Port Vale | 0–0 | Wigan Athletic | 7,348 | Vale Park | Elliot Bell | Match Report | |
| | | | | | | | | | | |
| 7 | Tue | 2026-04-21 | 19:45 | Stevenage | 1–0 | Barnsley | 3,620 | The Lamex Stadium | Ben Speedie | Match Report | |
| | | | | | | | | | | |
| 36 | Tue | 2026-04-21 | 19:45 | Stockport | 0–1 | Mansfield Town | 8,763 | Edgeley Park | Scott Jackson | Match Report | |
| | | | | | | | | | | |
| 40 | Tue | 2026-04-21 | 19:45 | Rotherham United | 0–2 | Luton Town | 7,497 | AESSEAL New York Stadium | Sam Mulhall | Match Report | |
| 40 | Tue | 2026-04-21 | 19:45 | Bradford City | 1–1 | Plymouth Argyle | 18,475 | University of Bradford Stadium | Jamie O'Connor | Match Report | |
| 40 | Tue | 2026-04-21 | 19:45 | Doncaster Rovers | 0–2 | Lincoln City | 10,241 | Eco-Power Stadium | Seb Stockbridge | Match Report | |
| | | | | | | | | | | |
| 41 | Wed | 2026-04-22 | 19:45 | Cardiff City | 1–0 | Port Vale | 18,950 | Cardiff City Stadium | David Rock | Match Report | |
| | | | | | | | | | | |
| 45 | Sat | 2026-04-25 | 12:30 | Stockport | 3–1 | Peterborough United | 9,432 | Edgeley Park | Jacob Miles | Match Report | |
| 45 | Sat | 2026-04-25 | 12:30 | Doncaster Rovers | 1–1 | Stevenage | 8,770 | Eco-Power Stadium | Martin Coy | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Blackpool | 1–0 | Leyton Orient | 10,735 | Bloomfield Road Stadium | Darren Drysdale | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Lincoln City | 4–3 | Wycombe Wanderers | 10,125 | LNER Stadium | Scott Oldham | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Cardiff City | 5–1 | Northampton Town | 23,223 | Cardiff City Stadium | Edward Duckworth | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Burton Albion | 1–1 | Exeter City | 5,729 | Pirelli Stadium | Elliot Bell | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Wigan Athletic | 0–1 | AFC Wimbledon | 10,179 | The Brick Community Stadium | Matthew Corlett | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Bradford City | 1–1 | Bolton Wanderers | 23,732 | University of Bradford Stadium | Seb Stockbridge | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Plymouth Argyle | 2–1 | Port Vale | 16,129 | Home Park | Isaac Searle | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Huddersfield Town | 1–4 | Mansfield Town | 17,843 | Accu Stadium | Ross Joyce | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Luton Town | 2–1 | Barnsley | 11,781 | Kenilworth Road Stadium | Harry Wager | Match Report | |
| 45 | Sat | 2026-04-25 | 15:00 | Rotherham United | 1–1 | Reading | 8,029 | AESSEAL New York Stadium | John Brooks | Match Report | |
| | | | | | | | | | | |
| 40 | Tue | 2026-04-28 | 19:45 | Peterborough United | 0–0 | Mansfield Town | 6,040 | Weston Homes Stadium | Lewis Sandoe | Match Report | |
| 40 | Tue | 2026-04-28 | 19:45 | Northampton Town | 0–1 | Barnsley | 5,143 | Sixfields Stadium | Jamie O'Connor | Match Report | |
| | | | | | | | | | | |
| 43 | Tue | 2026-04-28 | 19:45 | Stockport | 1–2 | Port Vale | 8,884 | Edgeley Park | Alex Chilowicz | Match Report | |
| | | | | | | | | | | |
| 46 | Sat | 2026-05-02 | 15:00 | Wycombe Wanderers | 3–2 | Rotherham United | 5,568 | Adams Park | Harrison Blair | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Mansfield Town | 5–4 | Cardiff City | 9,401 | One Call Stadium | Charles Breakspear | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Stevenage | 1–0 | Wigan Athletic | 6,671 | The Lamex Stadium | Matthew Russell | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Northampton Town | 2–3 | Plymouth Argyle | 7,131 | Sixfields Stadium | Ben Speedie | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | AFC Wimbledon | 0–4 | Huddersfield Town | 8,784 | The Cherry Red Records Stadium | George Laflin | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Leyton Orient | 2–2 | Burton Albion | 8,544 | The BetWright Stadium | Paul Howard | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Bolton Wanderers | 2–3 | Luton Town | 25,555 | Toughsheet Community Stadium | Carl Brook | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Exeter City | 1–2 | Bradford City | 8,015 | St James Park | Oliver Yates | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Peterborough United | 1–3 | Doncaster Rovers | 10,192 | Weston Homes Stadium | Edward Duckworth | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Barnsley | 1–3 | Stockport | 12,940 | Oakwell Stadium | Seb Stockbridge | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Port Vale | 0–2 | Lincoln City | 8,980 | Vale Park | Andrew Humphries | Match Report | |
| 46 | Sat | 2026-05-02 | 15:00 | Reading | 0–1 | Blackpool | 12,602 | Select Car Leasing Stadium | Elliot Bell | Match Report | |
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